| |
| Feature |
Position |
Benefit |
| |
| GAL1 promoter |
Bases 1-451 |
Allows inducible expression of genes cloned into pYES-DEST52 |
| |
| T7 promoter |
Bases 475-494 |
Allows in vitro transcription in the sense orientation |
| |
| attR1 recombination site |
Bases 511-635 |
Allows recombinational cloning of the gene of interest from an entry clone |
| |
| Chloramphenicol resistance gene, CmR |
Bases 771-1430 |
Allows counterselection of expression clones |
| |
| ccdB gene |
Bases 1772-2077 |
Allows negative selection of expression clones |
| |
| attR2 recombination site |
Bases 2118-2242 |
Allows recombinational cloning of the gene of interest from an entry clone |
| |
| V5 epitope* |
Bases 2265-2306 |
Allows detection of the fusion protein by the Anti-V5 antibodies |
| |
| Polyhistidine (6xHis) region |
Bases 2316-2333 |
Allows purification of your fusion protein on metal-chelating resin (e.g. ProBond); allows detection of the recombinant protein by the Anti-His(C-term) antibodies |
| |
| CYC1 transcription termination signal |
Bases 2366-2619 |
Allows efficient termination and stabilization of mRNA |
| |
| pUC origin |
Bases 2803-3476 |
Allows high copy number replication and growth E.coli |
| |
| Ampicillin (bla) resistance gene |
Bases 3321-4481 (Complementary) |
Allows selection of transformants in E.coli |
| |
| URA3 gene |
Bases 4499-5606 (Complementary) |
Allows selection of yeast transformants in uracil-deficient medium |
| |
| 2u origin |
Bases 5610-7081 |
Allows maintenance and high copy replication in yeast |
| |
| f1 origin |
Bases 7149-7604 (Complementary) |
Allows rescue of single-stranded DNA |
| |
