Part 1:  Isolation of Luminescent Bacteria

Table 2.  Results Observed Daily After Incubation of Squid Overnight

Day 1 after incubation of squid	Day 2	Day 3	Day 4	Day 5
-No luminous colonies were visible on squid Figure 2.  Squid after incubation overnight at four degrees celcius.	-Several luminous colonies observed on NaCl Agar -Other bacterial colonies observed growing on plates -No colonies present on squid or luminous media	-Isolated luminous coloines appeared on NaCl agar -No other bacterial colonies present -No colonies present on squid or luminous media -No growth observed in seawater complete liquid medium Figure 3.  Initial isolated luminous colonies on NaCl Agar incubated at room temperature.	-Growth of isolated organism was maintained on NaCl Agar -No colonies present on squid or luminous media -Slight glow observed in seawater complete liquid medium Figure 4.  Maintained growth of luminous bacteria on NaCl Agar at room temperature.  Isolated colonies present.	-Continued presence of luminous colonies on NaCl Agar -No colonies present on squid or lumionus media -Growth throughout seawater complete liquid medium Figure 5.  Bacteria glowing in seawater complete liquid medium at room temperature.

Part 2:  Characterization of Luminescent Bacteria

Table 3.  Tests Performed for Characterization and Interpretation of Results obtained

Test Performed	Results Obtained	Interpretation
Gram Stain	Figure 6. Gram stain performed with colonies that were growing on NaCl Agar for 24 hours at room temperature. Pink, short plump rods.	Gram negative, short, plump, bacillus
Motility Stab	Figure 7.  Motility stab performed on mannitol, enriched with NaCl,  incubated for two days at room temperature.  Invisible stab line and growth throughout.	Motile
Wet Mount	Movement noted	Motile
Oxidase Test	No change in color	Oxidase negative
Carbohydate utilization of Glucose and Mannitol	Figure 8. Mannitol tube (left) showing no color change and glucose tube (right) showing color change and no gas evolution. Tubes incubated overnight at room temperature.	Fermentation of glucose with acid end products and no gas evolution. Did not ferment mannitol.  Facultative anaerobe.

---

Return Home                                         Back to Methods                                       Discussion