BSCI 415

Laboratory in Molecular Genetics

Fall 2010

LABORATORY SCHEDULE:

Aug 31    Lab 1   Introduction- Model genetic systems

Sept 2     Lab 2   Mutagenesis 1: Plant mutagenized Arabidopsis population

Miniproject- Reverse Genetics: plant seed of gene knock-outs

Sept 7     Lab 3   Mutagenesis 2: Mutagenize C. elegans

   Read paper: The art and design of Genetic Screens: C. elegans

http://www.nature.com/nrg/journal/v3/n5/pdf/nrg794.pdf

Sept 9     Lab 4   Mutagenesis 3:  Removal of M0 from C. elegans plates

                           Paper discussion- Screen for small body size

http://www3.interscience.wiley.com/cgi-bin/fulltext/104077051/PDFSTART

Sept 14   Lab 5   Mutagenesis 4:  Screening M2 C. elegans for mutations       

Sept 16   Lab 6   Mutagenesis 4: Screening M2 C. elegans for mutations

Sept 21   Lab 7   PCR cloning 1: Collection of cheek cells

Mutagenesis- transfer selfed mutants- check on phenotypes

Sept 23   Lab 8   PCR cloning 2&3 Preparation of genomic DNA & PCR amplification

                                       Paper discussion CYP2D6 phenotypes

Homework #1 due- Wormbase

Sept 28   Lab 9   PCR cloning 4: Gel electrophoresis of PCR products

                           Transformation 1: Transformation of Arabidopsis plants

                           Mapping 1: Plant M2 seeds

Sept 30   Lab 10 Mutagenesis 5:  Screen Arabidopsis

Homework #2- C. elegans papers

Oct 5      Lab 11 PCR cloning 5: Cleanup of PCR products for sequencing

Oct 7      Lab 12 Mutagenesis 6: Crossing mutants for complementation

Homework #3 Arabidopsis database

Oct 12    Lab 13 Mutagenesis 7: Analysis of phenotypes from crosses

                                                    Set up additional test crosses

Oct 14    Lab 14 PCR cloning 6: Computer analysis of PCR sequences

Homework #4 CYP2D6 SNP and allele database

Oct 19    Lab 15 Transformation 2: Collection of T1 seeds

Oct 21    Lab 16 PCR cloning 7: Class comparison of PCR sequences

Quiz 1

Oct 26    Lab 17 Transformation 2: Sterilization and plating of T1 seed onto selective media

Oct 28    Lab 18 Mutagenesis 8: Analysis of phenotypes from crosses

Report on PCR cloning project due

Nov 2     Lab 19  Mapping 2: Screen seedlings for phenotypes to select mutants/wt

Nov 4     Lab 20   Paper discussion-Plant Transformation

Homework # 5 Arabidopsis Mapping and Gene Expression

Nov 9     Lab 21 Transformation 3: GUS staining of T1 seedlings

Nov 11   Lab 24 Transformation 4: Photographing GUS staining patterns

Nov 16   Lab 22 Mapping 3: Genomic DNA prep from plants

Nov 18   Lab 23 Mapping 4: PCR analysis of InDel Markers  

Nov 23   Lab 25 Mapping 5: Gel electrophoresis of InDel Markers

Report on transformation project due

Nov 30   Lab 26   Mapping 6: Database analysis of mapping

                                       PCR second set of CAPS/SSLP markers

Dec 2      Lab 27   Mapping 6: Database analysis of CAPS mapping

                                       Electrophoresis of SSLP, digestion of CAPS

Dec 7      Lab 28 Mapping 7:  Electrophoresis of CAPS markers

                           Report on mutagenesis due

Dec 9      Lab clean-up

Lab report on mapping project due

Dec 16 (Thursday)          Final exam  10:30-12:30