Daniel C. Stein

Professor

Dr. Stein's research focuses on the mechanisms by which the human pathogen, Neisseria gonorrhoeae, is able to synthesize and regulate the expression of its outer membrane antigens, and how changes in the expression of these components effect disease outcome. Students use molecular genetic, cellular, immunological and biochemical techniques to further our understanding of gonococcal disease. 

Selected publications

Banerjee, A., R. Wang, S. Uljohn, P. A. Rice, E. C. Gotschlich and D. C. Stein. 1998. Identification of the gene (lgtG) encoding the lipooligosaccharide β chain synthesizing glucosyl transferase from Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. 95:10872-10877

Piekarowicz, A., M. Gołaszewska, A. Olubunmi Sunday, M. Siwińska and D. C. Stein. 1999. The HaeIV restriction modification system of Haemophilus aegyptius is encoded by a single polypeptide. J. Mol. Biol. 293:1055-1065.

Song, W., M. Lang, R Chen and D.C. Stein. 2000. Role of Lipooligosaccharide in Opa-independent Invasion of Neisseria gonorrhoeae into Human Epithelial Cells. J. Exp. Med.191:949-959.

Ram, S., A.D. Cox, J. C. Wright, U. Vogel, S. Getzlaff, R. Boden, J. S. Plested, S. Meri, S. Gulati, D. C. Stein, J. C. Richards, E. R. Moxon and P. A. Rice. 2003. Neisserial lipooligosaccharide is a target for complement C4b: inner core phosphoethanolamine residues define C4b linkage specificity. J. Biol. Chem. 278:50853-62.

Braun, D. C and D. C. Stein. 2004. The lgtABCDE gene cluster, involved in LOS biosynthesis in Neisseria gonorrhoeae, contains multiple promoter sequences. J. Bacteriol. 186:1038-1049.

O’Connor, E. T., A. Piekarowicz, K. V. Swanson, J. M. Griffiss, and D. C. Stein. 2006. Biochemical analysis of Lpt-3, a protein responsible for phosphoethanolamine addition to lipooligosaccharide of pathogenic Neisseria. J. Bacteriol.188:1039-1048.

Patrone, J.B., S. E. Bish and D. C. Stein. 2006. TNFa-independent IL-8 Expression: Alterations in Bacterial challenge Dose Cause Differential Human Monocytic Cytokine Response.  J. Immunol. 177:1314-1322

Patrone JB, Stein DC. 2007.  Effect of gonococcal lipooligosaccharide variation on human monocytic cytokine profile. BMC Microbiol. 7:7.

Piekarowicz A, Klyz A, Majchrzak M, Adamczyk-Poplawska M, Maugel TK, and Stein DC. 2007.  Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage. BMC Microbiol. 5:66.

O’Connor, ET, KV Swanson, H Cheng, K Fluss, JM Griffiss, and DC Stein.  Structural Requirements for Monoclonal Antibody 2-1-L8 Recognition of Neisseria Lipooligosaccharides. In press: Hybridoma