| |
| Feature |
Position |
Benefit |
| |
| T35S Terminator |
Bases 18-243 |
Terminator of 35S |
| |
| attR2 recombination site |
Bases 253-377 (Complementary) |
Allows recombinational cloning of the gene of interest from an entry clone |
| |
| ccdB gene |
Bases 418-723 (Complementary) |
Allows negative selection of expression clones |
| |
| Chloramphenicol resistance gene, CmR |
Bases 1065-1724 (Complementary) |
Allows counterselection of expression clones |
| |
| attR1 recombination site |
Bases 1833-1957 (Complementary) |
Allows recombinational cloning of the gene of interest from an entry clone |
| |
| Egfp |
Bases 1964-2680 (Complementary) |
enhanced green-fluorescent protein |
| |
| p35S |
Bases 2702-3729 (Complementary) |
35S promoter plus a part of omega leader |
| |
| RB |
Bases 3749-3948 |
|
| |
| Sm/SpR |
Bases 8852-10101 (Complementary) |
|
| |
| LB |
Bases 10107-10439 |
Allows selection of transformants in E.coli |
| |
| Kan |
Bases 10443-11839 (Complementary) |
|
| |
| |
| Feature |
Position |
Benefit |
| |
| T35S Terminator |
Bases 18-243 |
Terminator of 35S |
| |
| Egfp |
Bases 247-966 (Complementary) |
enhanced green-fluorescent protein |
| |
| attR2 recombination site |
Bases 974-1098 (Complementary) |
Allows recombinational cloning of the gene of interest from an entry clone |
| |
| ccdB gene |
Bases 1139-1444 (Complementary) |
Allows negative selection of expression clones |
| |
| Chloramphenicol resistance gene, CmR |
Bases 1786-2445 (Complementary) |
Allows counterselection of expression clones |
| |
| attR1 recombination site |
Bases 2554-2678 (Complementary) |
Allows recombinational cloning of the gene of interest from an entry clone |
| |
| p35S |
Bases 2706-3733 (Complementary) |
35S promoter plus a part of omega leader |
| |
| RB |
Bases 3753-3952 |
|
| |
| Sm/SpR |
Bases 8856-10105 (Complementary) |
|
| |
| LB |
Bases 10111-10443 |
Allows selection of transformants in E.coli |
| |
| Kan |
Bases 10447-11843 (Complementary) |
|
| |
