Micrographs of mature spermatozoids of the water fern Marsilea vestita.

The left-most micrograph depicts a paraformaldehyde-fixed spermatozoid, viewed with phase contrast microscopy. (bar = 50 um). The center micrograph depicts staining in the same spermatozoid with a mixture of anti-alpha- and anti-beta-tubulin antibodies, and fluorescence from a Texas Red-conjugated secondary antibody. The micrograph on the right shows the same cell, stained with Hoescht 33342, to reveal nuclear DNA. (Cells were prepared and photos were taken by Peter Hart)

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