Micrographs of mature spermatozoids of the water fern Marsilea vestita.



The left-most micrograph depicts a paraformaldehyde-fixed spermatozoid, viewed with phase contrast microscopy. (bar = 50 um). The center micrograph depicts staining in the same spermatozoid with a mixture of anti-alpha- and anti-beta-tubulin antibodies, and fluorescence from a Texas Red-conjugated secondary antibody. The micrograph on the right shows the same cell, stained with Hoescht 33342, to reveal nuclear DNA. (Cells were prepared and photos were taken by Peter Hart)


Click here to return to S. Wolniak's HomePage