Protocol for autoclaving glassware

  1. Dirty glassware needs to be thoroughly scrubbed out using Liquinox soap.
  2. Then rinse glassware 3x with hot tap water.
  3. Finally rinse 2x with deionized water.
  4. Place on drying rack until completely dry.
  5. For bottles, loosely screw on cleaned caps. Glassware without tops needs to have a piece of aluminum foil formed over the top opening.
  6. A piece of autoclave tape then needs to be placed over the cap or the aluminum foil.
  7. Glassware can then be loaded into one of the autoclave trays and placed into the autoclaver.
  8. Empty glassware should be autoclaved on dry setting.
  9. If there are any liquids in the batch, default to the liquids setting. This will avoid any drying cycle which would evaporate some of the solutions.

Note: All glassware can be autoclaved, as well as most plastic ware. Check that the plastic is made from polystyrene or polypropylene. Do not autoclave polyethylene as it will melt. As a general rule if the plastic is slightly opaque and hard then it can be safely autoclaved, if it is flimsy it probably can not. See Nalgene for more information on autoclaving plastics.