Tomato Juice Agar (1)
                           40 ml          Tomato Juice
                           10 g.           Peptonized Milk
                           10 g.           Pancreatic Digest of Casein
                           12 g.           Agar

                           pH ~ 6.1 +/- 0.2 at 25^oC

                    i)   Added dH₂0 to 1 Liter.

                    ii)  Dissolved media components in large flask.

                    iii) Autoclaved media.

                    iv) Poured media in petri dishes and allowed to solidify.
 

                    Tomato Juice Agar (2)
                          100 ml          Tomato Juice
                          100 g.           Skim Milk
                              5 g.           Yeast Extract
                            12 g.           Agar

                   i)   Added skim milk powder to flask then fillled to 500 ml with dH ₂0.

                   ii)  Added remaining ingredients to a separate flask and filled to 500 ml with                                     dH ₂ 0.

                   iii) Autoclaved flasks separately.

                   iv) Combined two flasks of media.

                   v)  Poured media in petri dishes and allowed to solidify.

                   i)   Inoculated tomato juice agar (1) and tomato juice agar (2)                                                         plates with fresh whole milk using streak plate technique.

                   ii)   Incubated at 37^oC for 48 hours.

                   iii)  Isolated and restreaked colonies with characteristic morphologies on T-Soy 
                        agar.

                  i) Performed the following procedures for organism identification:
                                   - Gram stain 
                                   - Capsule stain 
                                   - Endospore stain
                                   - Motility stab agar incubated for 48 hours at 37^oC
                                   - Oxidase/catalase assay
                                   - Incubation for 48 hours at 37^oC in T-Soy Broth
                                   - Incubation for 48 hours at 37^oC on MacConkey’s medium
                                   - Incubation for 48 hours at 37^oC on sheep's blood agar medium
                                   - Incubation for 48 hours at 37^oC in anaerobe chamber
                                   - Carbohydrate Utilization (Glucose and Lactose) 
                                   - 48 hour incubation in adverse temperature conditions 
                                      (4^o C and 55 ^oC)