Results[3]

Results of Diagnostic
Assays Performed on Isolate


ASSAY	RESULT	NOTES
Growth on Tomato Juice Agar (1)	Figure 2. Colonial growth on tomato juice agar (1) incubated at 37^oC for 48 hours.	Colony Morphology: Shape: circular Margin: entire Elevation: convex Size: pinpoint Texture: smooth Appearance: dull Pigmentation: nonpigmented Optical Property: opaque
Growth on Tomato Juice Agar (2)	Figure 3. Colonial growth on tomato juice agar (2) incubated at 37^oC for 48 hours.	Colony Morphology: Shape: circular Margin: entire Elevation: convex Size: pinpoint Texture: smooth Appearance: dull Pigmentation: nonpigmented Optical Property: opaque
Growth on T-Soy Agar	Figure 4. Colonial growth on T-soy incubated at 37^o C for 48 hours.	Colony Morphology: Shape: circular Margin: entire Elevation: convex Size: pinpoint Texture: smooth Appearance: dull Pigmentation: nonpigmented Optical Property: opaque
Gram Stain	Figure 5. Gram stain of isolate colony from T-Soy agar. (1000x magnification)	Gram positive cocci observed
Endospore Stain	Figure 6. Endospore stain of isolate colony from T-soy agar. (1000x magnification)	No sporulation observed. Cells stained with safranin.
Capsule Stain	Figure 7. Capsule stain of isolate colony from T-soy agar. (1000x magnification)	No capsules observed.
MacConkey's Agar Growth	Figure 8. Growth on MacConkey's agar incubated at 37^o C for 48 hours.	No growth observed.
Sheep's Blood Agar Growth	Figure 9. Colonial growth on sheep's blood agar incubated at 37^oC for 48 hours.	Growth observed, gamma-hemolysis pattern.
Anaerobic Chamber Growth	Figure 10. Colonial growth on T-soy agar incubated at 37^o C for 48 hours in an anaerobic chamber.	Colony Morphology: Shape: circular Margin: entire Elevation: convex Size: pinpoint Texture: smooth Appearance: dull Pigmentation: nonpigmented Optical Property: opaque
Oxidase Assay	No color change on swab.	Oxidase negative.
Catalase Assay	Slight bubble formation.	Catalase negative.
Carbohydrate Utilization Tests	Figure 11. Innoculated glucose tube incubated at 37^o C for 48 hours. Figure 12. Inoculated lactose tubes incubated at 37^o C for 48 hours. Environmental isolate on left . E. Coli control on right.	Color change and bubble in Durham tube observed (Figure 11). No color change or bubbles observed in lactose tube innoculated with environmental isolate. Color change and bubble formation in E. Coli control tube. (Figure 12)
Motility Stab	Figure 13. Inoculated motility stab agar incubated at 37^o C for 48 hours.	Growth confined to stab line.
Growth in 4^oC	No growth observed.	No growth observed.
Growth in 55^oC	No growth observed.	No growth observed.
Growth in T-Soy Broth	Figure 14. Simple stain of enviromental isolate extracted from T-soy broth. (1000x magnification)	Cellular Arrangement: Long strands/chains of cocci

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