Eukaryotic Cell Biology (BSCI 421) | University of Maryland

Alberts et al., Molecular Biology of the Cell, 4th Edition. Garland Publishing. New York.
Wolniak et al., 2003. Eukaryotic Cell Biology - A Laboratory Manual 8th Edition

Eukaryotic Cell Biology
Lecture Schedule

Date	Topic	Readings

Jan 27	Introduction to Cell Biology Purpose of course/historical perspective Minimal requirements for life Constituents of the cell Origins of cells Prokaryotes vs eukaryotes The minimal cell Parts of the eukaryotic cell	Ch. 1
Jan 29	Basic Biochemistry	Ch. 2,3,4
Feb 3-10	Techniques to study cells	Ch. 8,9
Feb 12	The Plasma Membrane Types of movement Energetics of movement	Ch. 10
Feb 17	Membrane Transport Processes Types of movement Energetics of movement	Ch. 11
Feb 19	Macromolecular Transport Endocytosis Exocytosis	Ch. 13
Feb 24-26	Internal Membrane Systems Separation of metabolic activities The Endoplasmic Reticulum Signal hypothesis-translation on RER Vectorial transport/discharge	Ch. 12,13
Mar 2	The Golgi Apparatus Protein processing and sorting	Ch. 13
	Lysosomes and Vacuoles	Ch. 13
	Peroxisomes, Glyoxysomes and Microbodies Composition and functions	Ch. 12
Mar 4	Midterm Examination I
Mar 9	Organelles with double membranes, Nucleus, mitochondria, plastids	Ch. 14
Mar 11	The Nucleus Chromatin organization, nucleosomes Nuclear envelope, nuclear pores Gene regulation, transcription	Ch. 4,5,6,12
Mar 16-18	The Nucleolus; Ribosomes rRNA, precursor and processing Ribosomal organization Translation	Ch. 6
Mar 23-25	No Lecture - Spring Break
Mar 30	Mitochondria Organization, composition Chemiosmosis, electron transport, ATP formation	Ch. 14
Apr 1-6	Chloroplasts (and other plastids) Organization, composition Photosynthesis Carbon fixation	Ch. 14
	Inheritance in Mitochondria and Chloroplasts Similarities/differences between organelle DNA and nuclear or prokaryotic genomes	Ch. 14
	Post-translational import of nuclear gene products The evolution of mitochondria and plastids	Ch. 12
Apr 8	Midterm examination II
Apr 13-20	The Cytoskeleton Microtubules and microfilaments	Ch. 16
Apr 22-27	Cell Motility Life with a low Reynolds Number Paradigms for movement (flagellar axoneme, skeletal muscle sarcomere) Cytoplasmic streaming, wall formation	Ch. 16
Apr 27-29	Cell Replication, Cell Cycle Mitosis, meiosis Centrosomes and centrioles Cytokinesis	Ch. 17,18
May 4-11	Cell Signalling Principles of signal reception and transduction Regulatory cascades - kinases and phosphatases	Ch. 15
May 18	FINAL EXAMINATION	10:30 AM - 12:30 PM

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Course requirements:	Midterm exams	Thursday, March 4	20 % of grade
		Thursday, April 8	20 % of grade
	Final	Wednesday, May 18	25 % of grade
	Laboratory (room 1171 Pl Sci)		35 % of grade
	(lab practical, lab notebook, write-ups the TAs will provide details on lab points)
Office hours:	By appointment

Dates		Special Events	Lab Exercise

Feb	3-5		1. Cell Diversity
	10-12		2. Physiology of Fertilization in Sea Urchins
	17-19		3. Parameters Affecting Taq Activity in PCR
	24-26		4. The Plasma Membrane Permeability in Erythrocytes
Mar	2-4	6: MIDTERM I	5a. Chloroplast Isolation and Fractionation
	9-11		5b. Electrophoretic Analysis of Chloroplast Proteins
	16-18		6a. Spatial Distributions of Organelles
	23-25	Spring Break	-------------------
Mar	30- Apr 1		6b Experimental Manipulation of Organelle Distribution
	6-8	10: MIDTERM II	7. Organization of the Cytoskeleton
	13-15		8a. Cell Cycle Dynamics I - Growth Factor Receptors
	20-22		8b. Cell Cycle Dynamics II - Regulation
Apr	27- 29		Laboratory Practical Examination
			Notebooks and reports are due by 5/6

Eukaryotic Cell Biology
4 credit hours

Textbook | Course Requirements | Office Hours | Lecture Schedule | Laboratory Schedule

Eukaryotic Cell Biology 4 credit hours

Textbook | Course Requirements | Office Hours | Lecture Schedule | Laboratory Schedule

Eukaryotic Cell Biology
4 credit hours