PLANT
PHYSIOLOGY OUTLINE |
Dr. H Sze |
|
FALL 2008
|
Lec. # 13: Photosynthesis
1: light reactions |
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OVERALL RX: CO2 + 2H2O + light--> [CH2O] + H2O + O2
I. Overview of Photosynthesis: 4 STAGES:
1. LIGHT ABSORPTION: Electrons are pulled from water,
and O2 is evolved. (LIGHT RX)
2. ELECTRON TRANSPORT: NADPH FORMED
(LIGHT RX)
3. GENERATION OF ATP
(LIGHT RX)
4. CONVERSION OF CO2 INTO CARBOHYDRATES
(Carbon RX)
II. FORMATION and Structure OF THE CHLOROPLAST
Outer membrane Inner membrane
Thylakoid
Stroma
Pigments are organized into light-harvesting complexes
(LHC) on the thylakoid membrane.
CHLOROPHYLL
A, major photosynthetic pigment
CHL B, accessory pigment
CAROTENOIDs, accessory pigment
III. LIGHT ABSORPTION BY PIGMENTS:
How is light absorbed?
What does absorbed light do?
a. PROPERTIES OF
LIGHT
b. CHLOROPHYLL ABSORBS A PHOTON, TRANSFERS EXCITATION
ENERGY AND
Reaction Center CHL GIVES UP AN ELECTRON
A) Excitation Energy Transfer: Light energy absorbed by pigments is transferred (funneled) to neighboring pigments and to a reaction center pigment Chl a.
b) Photochemical reaction: The reaction center pigment
chl a gives up an electron. i.e. becomes oxidized.
[I.E. Photochemistry refers to the photo-oxidation.]
PSII: Rx center P680 gives up an electron to
acceptor pheophytin. Water donates an electron to P680.
2 Chl a+
+ H2O --> 2 Chl a + 1/2 O2 + 2H+