BSCI 442  
PLANT PHYSIOLOGY  OUTLINE
Dr. H Sze
FALL 2008
Lec. # 13: Photosynthesis 1: light reactions
Slides
 

OVERALL RX: CO2 + 2H2O + light--> [CH2O] + H2O + O2

I.  Overview of Photosynthesis:  4 STAGES:

1. LIGHT ABSORPTION:  Electrons are pulled from water, and O2 is evolved.  (LIGHT RX)
2. ELECTRON TRANSPORT: NADPH FORMED                                           (LIGHT RX)
3. GENERATION OF ATP                                                                                 (LIGHT RX)
4. CONVERSION OF CO2 INTO CARBOHYDRATES                                     (Carbon RX)

II.  FORMATION and Structure OF THE CHLOROPLAST
            Outer membrane         Inner membrane
            Thylakoid                    Stroma

    Pigments are organized into light-harvesting complexes (LHC) on the thylakoid membrane.
           CHLOROPHYLL A, major photosynthetic pigment
            CHL B,  accessory pigment
            CAROTENOIDs, accessory pigment

III.  LIGHT ABSORPTION BY  PIGMENTS:
            How is light absorbed?
            What does absorbed light do?
 a.  PROPERTIES OF LIGHT

b.  CHLOROPHYLL ABSORBS A PHOTON, TRANSFERS EXCITATION ENERGY AND
Reaction Center CHL GIVES UP AN ELECTRON

A) Excitation Energy Transfer:  Light energy absorbed by pigments is transferred (funneled) to neighboring pigments and to a reaction center pigment Chl a.

b) Photochemical reaction:  The reaction center pigment chl a gives up an electron.  i.e. becomes oxidized.
[I.E. Photochemistry refers to the photo-oxidation.]

PSII:  Rx center P680 gives up an electron to acceptor pheophytin.  Water donates an electron to P680.
    2 Chl a+ + H2O --> 2 Chl a + 1/2 O2 + 2H+