Catalase is an enzyme found in most bacteria. It catalyzes the breakdown of hydrogen peroxide to release free oxygen.
2 H2O2 ---------> 2 H2O + O2
Procedure: Add one drop of H2O2 to a glass slide with a loopful of growth from each culture to be tested. The development of an immediate froth of bubbles is indicative of a positive catalase test. The test is performed on a blood-free medium.
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