BSCI 420/421/ZOOL708A
| Lectures: | MWF 9:00 A.M. PLS 1140 | Lecturer: Dr. Dennis Goode (and course coordinator) |
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| Laboratories: | 0101 Mon 1-5 PLS 1171 0102 Tue 1-5 PLS 1171 0103 Wed 1-5 PLS 1171 |
Office: PLS-3104 Phone: 405-6917 E-mail: dg0@umail.umd.edu |
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| Lab Coordinator: | Dr. Lisa Perrino - (lp53@umail.umd.edu) | ||
| Teaching Assistants: | Mr.Michael Dandenault - (md112@umail.umd.edu) Mr. Shane Hardin - (sh133@umail.umd.edu) Ms. Rana Khan - (ansari@wam.umd.edu) Mr. Jon Zmuda - (jz39@umail.umd.edu) |
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| Text: Alberts et al., Molecular Biology of the Cell, 3rd ed. (1994) Garland Press, Publisher |
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The Laboratory Manual will be copied by Reprographic Services and sold in the University Book Center, Student Union. We will announce in class when it is available.
Cell Biology is also available without the laboratory as BSCI 420 (3 cr) or ZOOL 708A (3 cr).
| Course Grading: | BSCI 421 | BSCI 420 | ZOOL 708A |
| Exam 1,2,3 | 300 points | 300 points | 300 points |
| Final Exam | 100 | 100 | 100 |
| Laboratory | 130 | ||
| Paper/Oral report | ________________________________________ 50 | ||
| TOTAL | 530 points | 400 points | 450 points |
Scale:
85 - 100% A
75 - 85% B
60 - 75 C
50 - 60% D
Below 50% F
This course is an advanced study of the molecular and structural bases of cell function with an emphasis on the integrated functions of whole cells and of organelles in eukaryotic cells. The prerequisites are: ZOOL 211 (BSCI 230), BIOL 222 (BSCI 222), and CHEM 233 or their equivalents. Students who have not had these prerequisites or their equivalents must obtain permission from D. Goode or drop the course. BCHM 461 can substitute for BSCI 230.
BSCI 421 CELL BIOLOGY Fall 1999
| Date | LECTURE TOPICS | Assignments (in Alberts et al.) | |
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INTRODUCTION TO CELL BIOLOGY |
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| Sept 1 | Introduction to Cell Biology: from molecules to prokaryotic cells | 3-16; review 41-136 | |
| Sept 3 | Eukaryotes and the origins of multicellularity | 17-39 | |
| Sept 8 | Ways of studying cells and cell contents | 139-157; 162-177 | |
| Sept 10 | Cell culture, fusion, and hybridomas | 157-162, 178-189 | |
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NUCLEAR STRUCTURE AND FUNCTION |
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| Sept 13 | Nuclear and chromosome structure | 335-342, 364-5 | |
| Sept 15 | Chromosome replication | 251-262, 356-364 | |
| Sept 17 | Chromatin structure and modifications | 342-356 | |
| Sept 20 | Molecular hybridization and Recombinant DNA techniques | 291-325 | |
| Sept 22 | RNA synthesis and processing | 365-371 | |
| Sept 24 | RNA processing , nucleoli and ribosome formation | 371-385 | |
| Sept 27 | Control of gene expression in eukaryotic cells | 400-437 | |
| Sept 29 | Control of gene expression in eukaryotic cells | " " | |
| Oct 1 | EXAM 1 (over lectures 1-12) | ||
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STRUCTURE AND FUNCTION OF MEMBRANES AND MEMBRANE-BOUND ORGANELLES |
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| Oct 4 | Membrane structure and function | 478-504 | |
| Oct 6 | Membrane proteins and transport | 505-522 | |
| Oct 8 | Compartments and information flow between nucleus and cytoplasm | 551-568 | |
| Oct 11 | Endoplasmic reticulum and protein synthesis | 577-594 | |
| Oct 13 | Vesicular traffic: Golgi and lysosomes | 599-618 | |
| Oct 15 | Secretion | 626-636 | |
| Oct 18 | Endocytosis | 618-626 | |
| Oct 20 | Molecular mechanisms of vesicular transport | 636-647 | |
| Oct 22 | Mitochondrial and chloroplast genomes and genesis | 568-574, 684-687,700-717 | |
| Oct 25 | Cell to cell junctions | 950-963 | |
| Oct 27 | Cell to cell adhesion and extracelular matrix | 963-1000 | |
| Oct 29 | EXAM 2 (over lectures 14-24) | ||
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COMMUNICATION BETWEEN CELLS |
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| Nov 1 | Cell signaling | 721-734 | |
| Nov 3 | Second messengers | 734-743 | |
| Nov 5 | Protein kinases | 202-205, 743-758,771-773 | |
| Nov 8 | Growth factors signal systems | 760-770 | |
| Nov 10 | Germ cells and fertilization | 1021-1034 | |
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CELL MOTILITY |
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| Nov 12 | The Cytoskeleton and intermediate filaments | 788-802 | |
| Nov 15 | Microtubule structure and assembly | 803-812 | |
| Nov 17 | Microtubule-based cell motility | 813-820 | |
| Nov 19 | Actin and myosin | 821-842 | |
| Nov 22 | Actin-myosin based cell motility | 208-210, 843-858 | |
| Nov 24 | EXAM 3 (over lectures 26-35) | ||
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CELL PROLIFERATION AND CANCER |
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| Nov 29 | Cell cycle and its control | 864-890 | |
| Dec 1 | Cell division controls in multicellular animals | 891-906 | |
| Dec 3 | Initiation of mitosis | 911-929 | |
| Dec 6 | Mechanisms of anaphase and cytokinesis | 929-943 | |
| Dec 8 | Cancer | 1255-1272 | |
| Dec 10 | Oncogenes and cancer | 1273-1286, 1290-1291 | |
| Dec 13 | Cell and tissue renewal, Apoptosis | 1142-1156, 1173-1179 | |
| Dec 17 | FINAL EXAM 1:30 - 3:30 PM in PLS 1140 (100 points - over lectures 37-43) |
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General information regarding conduct of the course:
A. Examinations:
Examinations will consist primarily of questions requiring short answers, essay answers, or problem solving and will cover lecture material and material in the required text readings. We will follow the Student Honor Code as printed in the catalog, and dishonesty will not be tolerated.
Exams should be written legibly and in pen if a regrade is to be considered. A request for the regrading of an individual exam should be made in writing and should include a brief explanation of the reasons behind the request and documentation that your answer is correct (e.g., copy of page from text or other published source. Such a request must be submitted within one week after the return of the graded exam. Exam questions that were unreadable may not be submitted for additional points.
B. Worked missed because of absence:
Absence caused by religious observance or participation in university activity at the request of university authorities will be excused if the course coordinator is notified of the conflict ahead of time. Absence caused by illness may be excused if a verifiable letter from a physician indicating the nature of the illness and necessity of the absence is provided to the course coordinator. Please notify the coordinator before the exam if possible. Students missing a scheduled examination for any of the above reasons will be given the opportunity to take a make up examination covering the same material within a reasonable time.
If you miss a laboratory for any of the above reasons, notify your TA; you may attend a laboratory on an alternate day of the same week but can not make-up a lab at a later date. You may be allowed to attend an alternate lab section in the same week for other reasons with the advance permission of both lab TAs, but if all lab groups are full, it may not be possible for you to actually do the experiment.
C. The laboratory:
Because the laboratory is such an important part of this course, laboratory attendance is mandatory for students enrolled in 421. We expect you to arrive for each lab on time and well prepared to do the exercise. You will lose 10 points for each lab period missed or late without an acceptable excuse or prior arrangement to attend another section. Three unexcused absences will result in a failing grade in the laboratory portion of the course. Written reports summarizing the results of laboratory exercises will be required. Detailed guidelines regarding the reports will be discussed in lab. Although experiments are often done in groups, the writing of the reports must be individual effort. Laboratory reports are due at the beginning of the next regular laboratory meeting of your section (the numbered labs above; Lab 9 is due December 10, 11, or 12). Late reports will lose 5 points for each day late.
BSCI 421 LABORATORY SCHEDULE
Sept. 13-15 1. Cell diversity and structural organization
" 20-22 2. Effects of magnesium and nucleotides on DNA polymerase activity
" 27-29 3. a) Identification and localization of organelles
Oct. 4-6 b) Identification and localization of organelles
Oct. 11-13 4. The plasma membrane: Permeability of erythrocyte membranes
Oct. 18-20 5. a) Cell fractionation: Isolation of chloroplasts
" 25-27 b) Cell fractionation: Analysis of stromal and thylakoid proteins
Nov. 1-3 6. Localization and manipulation of microtubules and microfilaments
" 8-10 7. Physiology of fertilization in sea urchin eggs
" 15-17 8. a) Cell proliferation: The effect of cell density on DNA synthesis. Intro and set up cultures
" 22-24 b) Cell proliferation: Receptor Induction
" 29-D3 c) Cell proliferation: Incorporation of BrdU and cell counting
Dec. 6-8 Written reports of lab 8 (or any other lab of your choice) are due at 4 pm to your TA.
The laboratory score will be based on:
Three write-ups of labs 2-9 (30 points each) 90
(any three of your choice-whatever turns you on)
Three quizzes (10 points each) 30
TA overall evaluation points 10
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130