Journal of Virology, Dec. 1994, p.8466-8469
Effect of
Template Size on Accumulation of Defective Interfering RNAs in
Protoplasts
Chunxia Zhang and Anne E. Simon
Department of Biochemistry and Molecular Biology and Program in
Molecular and Cellular Biology, University of Massachusetts, Amherst,
Massachusetts 01003
Received 1 August 1994/Accepted 14 September 1994
A turnip protoplast system has been used to study the elects of
template size and sequence on the replication and/or stability of a
small defective interfering (DI) RNA associated with turnip crinkle
virus. Our results indicated that as little as a single base
difference in the size of the molecule in some regions, rather than
the specific sequence, affected the level of DI RNA accumulating in
protoplasts. |