Last Journal
HomeAbstract Topics
Next Journal

Journal of Virology, Dec. 1994, p.8466-8469

Effect of Template Size on Accumulation of Defective Interfering RNAs in Protoplasts


Chunxia Zhang and Anne E. Simon

Department of Biochemistry and Molecular Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts 01003

Received 1 August 1994/Accepted 14 September 1994

A turnip protoplast system has been used to study the elects of template size and sequence on the replication and/or stability of a small defective interfering (DI) RNA associated with turnip crinkle virus. Our results indicated that as little as a single base difference in the size of the molecule in some regions, rather than the specific sequence, affected the level of DI RNA accumulating in protoplasts.