Exercises
·Unix Introduction
·BLAST
·PERL
·Genbank
·BLAST, GCG
·GCG
·Seqlab
·Synthesis
·MSA
·Paup
·Phylogeny
·Examine


·An editor primer
·A GCG cheatsheet
·Flat2fasta homework
·Dynamic Programming homework
·High scoring words homework
·GCG homework
·Seqlab homework
·Mystery sequence homework
·Paup homework

A longer analysis


Below is a segment of sequence from an unsequenced Pyrococcus library created by a researcher here at the University of Maryland. Tell its story as completely as possible.
The story of this sequence will consist of a single map describing the sequence and up to three pages of text. Thus do not provide the outputs of the assorted programs used to test hypotheses, instead discuss hypotheses in the written text.
Here are some guidelines to help in creating a map:
Start codon:  An Octagon
Stop codon: *
Intron region: Connect exons with ^
Exon region: A box
A successful assignment will demonstrate an ability to test multiple hypotheses and interpret the data from the tests. Use the process describes by Platt in order to tell a complete story about the sequence.
For other ideas it may be useful to check out the Arabidopsis genome browser: http://atensembl.arabidopsis.info/Arabidopsis_thaliana_TIGR/
or the drosophila genome: http://www.ensembl.org/Drosophila_melanogaster/
ACGTCATGGTTAACGGCGACAAGCCTCCGGACGATTTTGATATTGAGATA
ATAGTTGCAAAGCCCAAGAGGTTTAGGATAAAACCAGGAATTTACCAGAT
GGCATGGCACCTTGTTTTCAAGGCTTATGGAGATGATGAGCTGATTAAAG
TTGGCTATGTAGTTGGCTTTGGGGAAAAGAACTCGCTCGGCTTTGGAATG
GTTAAAGTCGAGGGTGGTAAACGTGTATCGGGTGGAGTTAAAAGTAGAGT
TATAACTCCGGCGTTTATCCGAGGAGCAGACCAGAAGAAGGCGGAGTTGA
GGGTAGCGTTTACTAGAAGGAATAATTTATCTGCTAACTATCCTTCCTCT
TATGAGTCCTCTATCAGAGAGGTGATTTTTCTATGTTGAAATTTACTGGC
AACTGGTTCATAGATGCTGGCATTTTGGGGTTCGTGAATTTGATGGAAGA
GGTTTACGGTTGGGATTTGGAGGAGCTTCAGAGACATATCAAAGAAGAGC
CGGAGAAGGTCTATTATGGGTATTTTCCACTAGCTTACTTTTACAGTTTA
GCACCCAAGGGCCAAGAGAACAAAGGGCTTCTCTTACAGGCTATGCAAGA
AATAGAAACTTTTGAAGGAGACAAACATAAATTGCTCGAGCTCGTGTGG

Created: Wed Sep 15 00:58:22 EDT 2004 by Chuck Delwiche
Last modified: Fri Oct 29 16:22:45 EDT 2004 by Ashton Trey Belew.